Journal: Science Advances

Article Title: Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage

doi: 10.1126/sciadv.adf6600

Figure Lengend Snippet: Antibody information.

Article Snippet: Anti-CD3ε , Novus Biologicals NBP2-44880 RRID:AB_2923168 , WB: 1:1000.

Techniques:

Journal: Science Advances

Article Title: Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage

doi: 10.1126/sciadv.adf6600

Figure Lengend Snippet: Antibody information.

Article Snippet: Anti-CD3ε , Novus Biologicals NBP2-44880 RRID:AB_2923168 , WB: 1:1000.

Techniques:

Journal: Pathogens

Article Title: Methods of Identifying Gordonia Strains in Clinical Samples

doi: 10.3390/pathogens11121496

Figure Lengend Snippet: Gordonia species, the type strains of which were initially isolated from environmental samples. Some strains of these species are human or veterinary pathogens.

Article Snippet: According to the List of Prokaryotic Names with Standing in Nomenclature ( https://lpsn.dsmz.de/ (accessed on 30 October 2022)), the Gordonia genus currently consists of 53 species, four of which (“ Gordonia australis ”, “ Gordonia jacobaea ”, “ Gordonia pseudoamarae ”, “ Gordonia terrea ”) are not validated, and Gordonia nitida is a later heterotypic synonym of Gordonia alkanivorans [ ].

Techniques: Isolation, Environmental Sampling, Infection

Journal: Scientific Reports

Article Title: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

doi: 10.1038/s41598-021-82990-y

Figure Lengend Snippet: Effects of BKM120 and olaparib on the PI3K/Akt /FOXM1/Exo1 signaling pathway. Effects of BKM120, olaparib, and a combination of BKM120 with olaparib on the expressions of FOXO1, FoxO3a, FOXM1 and Exo1 in MDA-MB-231 cells analyzed by ( A ) RT-PCR and ( C ) western blots. ( E ) Effects of drugs on the phosphorylation of FOXO1 and FoxO3a analyzed by western blots. Relative intensity expression obtained from the corresponding ( B ) RT-PCR and ( E,F ) western blots. Error bars represent SEM from the mean of three separate experiments. * P < 0.05 and ** P < 0.01 compared to control, # P < 0.05 and ## P < 0.01 compared to 19.2 μΜ olaparib-treated group.

Article Snippet: FOXM1 siRNA, Exo1 siRNA and NC siRNA were brought from Santa Cruz biotechnology.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Phospho-proteomics, Expressing, Control

Journal: Scientific Reports

Article Title: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

doi: 10.1038/s41598-021-82990-y

Figure Lengend Snippet: The roles of FOXM1 and Exo1 in the repair of DNA DSBs. ( A ) The expression of BRCA1, BRCA2, Rad51 and Rad52 after silence the expression of FOXM1 and Exo1 analyzed by western blots. ( B ) Relative intensity expression obtained from the corresponding western blots. ( C ) Comet assay analysis of DNA damage after silence the expression of FOXM1 and Exo1. ( D ) Apoptosis of MDA-MB-231 cells measured by Annexin V-FITC and PI double staining after silence the expression of FOXM1 and Exo1. ( E ) Immunofluorescence analysis of γh2AX after silence the expression of FOXM1 and Exo1. Error bars represent SEM from the mean of three separate experiments. * P < 0.05 and ** P < 0.01 represent FOXM1 siRNA group compared to NC siRNA group, # P < 0.05 and ## P < 0.01 represent Exo1 siRNA group compared to NC siRNA group.

Article Snippet: FOXM1 siRNA, Exo1 siRNA and NC siRNA were brought from Santa Cruz biotechnology.

Techniques: Expressing, Western Blot, Single Cell Gel Electrophoresis, Double Staining, Immunofluorescence

Journal: Scientific Reports

Article Title: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

doi: 10.1038/s41598-021-82990-y

Figure Lengend Snippet: Mechanism for the synergistic effects of BKM120 and olaparib on the death of MDA-MB-231 cells. BKM120 directly induces DNA damage through increasing cellular ROS. Meanwhile, BKM120 downregulates the expression of PARP1 and PARP2 to synergy the inhibition of olaparib in the repair of DNA single damage. Moreover, BKM120 inhibits the HR repair of DNA double damage to sensitize cells to olaparib through regulation the PI3K/Akt/NFĸB/c-Myc pathway and PI3K/Akt/ /FOXM1/Exo1 pathway.

Article Snippet: FOXM1 siRNA, Exo1 siRNA and NC siRNA were brought from Santa Cruz biotechnology.

Techniques: Expressing, Inhibition

Journal: Scientific Reports

Article Title: BKM120 sensitizes BRCA-proficient triple negative breast cancer cells to olaparib through regulating FOXM1 and Exo1 expression

doi: 10.1038/s41598-021-82990-y

Figure Lengend Snippet: Primer sequences for the apoptosis factors in RT-PCR.

Article Snippet: FOXM1 siRNA, Exo1 siRNA and NC siRNA were brought from Santa Cruz biotechnology.

Techniques:

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: Gene expression profile in PLCε-silenced T24 cells and verification of selected DEGs. (A) RNA-seq mRNA expression data from Sanchez-Carbayo bladder dataset was used to compare PLCε expression between in BCa tumors (BCa) and normal bladder tissue (Bladder). (B) KEGG pathway enrichment analysis for the DEGs (> two-fold change and P < 0.05), the vertical axis was the pathway category, and the horizontal axis was the enrichment of pathways. (C) The significant GO category for indicated genes of mismatch repair between Ad-shPLCε and Ad-NC groups, the vertical axis was the GO terms, and the horizontal axis was the enrichment of GO, P value < 0.01 was used as a threshold to select significant GO categories. (D) Hierarchical clustering analysis of DEGs involved in DNA damage repair pathways. High expression levels are shown in green and low levels in red. (E, F) Detection gene levels of 4 selected DEGs by q-PCR in T24 and BIU-87 cells. The q-PCR data were normalized to the expression of β-actin (G) RNA-seq mRNA expression data from the TCGA to compare EXO1 expression between in BCa tumors (T) and their non-tumor counterparts (N). *P ≤ 0.05, **P ≤ 0.01, AND ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing, RNA Sequencing Assay

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: High expression levels of PLCε and EXO1 in BCa tissues. (A, B) Disease free survival curves of BCa patients according to EXO1 and ATM mRNA levels in TCGA. (C) Representative haematoxyl in and eosin (H&E) staining and IHC staining in 72 BCa tissue samples and 24 corresponding adjacent non-neoplastic (BN) samples. Magnification × 200. Bars = 150 μm. H&E staining of bladder tissues were showed (a and i). Representative IHC staining of different staining intensities used as criteria in staining scoring: none staining was observed in BN (b-d), positive staining in BCa (j-l). PLCε localized in cytoplasm. EXO1 and ATM protein localized in nucleus. Images in the frame of (a-d and i-l) were enlarged to (e-h and m-p). (D-F) Average staining scores for PLCε, EXO1, and ATM expression in bladder tissues. (G, H) The correlation with PLCε and EXO1 expression and EXO1 and ATM expression in bladder tissues samples was analyzed by Pearson analysis. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: Correlation between PLCε, EXO1 and ATM expressions and the corresponding clinicopathological parameter in BCa patients

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques:

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: A association between PLCε and EXO1 expression in BCa patients. A. PLCε and EXO1 protein expression in 24 BCa tissue samples (T) and 24 BN samples (N) were assayed by western blotting analysis. B, C. PLCε and EXO1 protein expression in tissues were quantified as mean optical density by Image J software. D, E. PLCε and EXO1 protein expression in tissues were assayed by Statistical analysis. F. Correlation curve of PLCε protein versus corresponding EXO1 by Statistical analysis. G. Western blotting analysis detected the expression of PLCε and EXO1 in two human BCa cell lines and SV-HUC-1 cells. H, I. Detection of PLCε and EXO1 by qPCR. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Western Blot, Software

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: PLCε depletion inhibit cell proliferation, induce G1 phase cell cycle arrest, apoptosis and cisplatin sensitivity. A. The T24 and BIU-87 cells were infected with adenovirus sh-PLCε, western blotting were detected the expression of PLCε. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX. C, D. Representative flow cytometry analysis of cell cycle phase distribution after 48 h of culture, compared to blank group. E-K. The T24 and BIU-87 cells were infected with vector-EXO1 for 72 h. E, F. Representative flow cytometry analysis of cell apoptosis after 72 h of culture in Ad-shPLCε or/and EXO1 groups. G, H. Clone formation analysis was employed to test the cell proliferation in Ad-shPLCε or/and EXO1 groups. I. IC50 values were measured by CCK-8 to compare the susceptibility of the three cell lines to cisplatin. J, K. IC50 values of T24 and BIU were measured by CCK-8 Analysis in Ad-shPLCε or/and EXO1 groups. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Infection, Western Blot, Expressing, Flow Cytometry, Plasmid Preparation, CCK-8 Assay

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX in sh-NC and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Translocation Assay, Infection, Plasmid Preparation, Western Blot, Expressing, Immunofluorescence, Staining

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: PLCε depletion enhanced the inhibitory effect of cisplatin on the growth of BCa cells. C-I. 8 μg/mL Cisplatin was treated in BCa cells for 48 h. A, B. Western blotting analysis showing the expressions of EXO1 and γ-H2AX stimulated by cisplatin at different concentrations (for 24 h) and different treat times (with 8 μg/mL) in BCa cells. C. Morphological change of bladder cancer cells with cisplatin or (and) Ad-shPLCε treatment. Magnification: 200×. Bars = 100 μm. D, E. Representative flow cytometry analysis of cell cycle phase distribution after 48 h of culture, compared to blank group. F. Western blotting detected the expression of cell cycle and proliferation related proteins. G, H. Clone formation analysis was employed to test the cell proliferation of BCa cell lines after treatment with cisplatin. I. Western blotting detected the expression of cell apoptosis related proteins. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Western Blot, Flow Cytometry, Expressing

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: Decreased levels of serum miR-145 in BCa patients and PLCε was a direct target of miR-145 in BCa cells. A. Comparison of miR-145 levels in three cell lines by qPCR. vs SV-HUC-1. B. Expression levels of miR-145 in T24 and BIU cells transfected with miR-145 mimics and NC. C, D. PLCε mRNA and protein expression levels in the BCa cell lines detected by qRT-PCR and western blotting after transfection with miR-145 mimics. E. Schematic representation of the predicted miR-145 binding sites in 3’UTR of PLCε. F. Wild-type or mutant 3’UTR of PLCε gene reporter plasmid was co-transfected with miR-145mimics or NC into T24, followed by luciferase reporter assays. vs miR-NC. G. Changes of PLCε expression analysised by qPCR in BCa cells after treating with cisplatin or (and) miR-145. H, I. 8 μg/mL Cisplatin was treated in BCa cells for 48 h. H. Viability of BCa cells with cisplatin or (and) miR-145 mimics treatment detected by CCK-8. I. Western blotting analysis showing the expression of PLCε, EXO1, and γH2AX after cisplatin or (and) miR-145 mimics treatment for 48 h. J. The serum miR-145 expression in 40 patients with BCa, 19 patients with urinary benign diseases (BBD) and 21 healthy controls (Normal) were obtained by qPCR. Expression levels of miR-145 were normalized to U6. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Binding Assay, Mutagenesis, Plasmid Preparation, Luciferase, CCK-8 Assay

Journal: American Journal of Cancer Research

Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

doi:

Figure Lengend Snippet: A mechanism for PLCε depletion-mediated cisplatin sensitivity of BCa. Schematic diagram describing the functional significance of PLCε in BCa cells. PLCε regulate EXO1 medicated-cell cycle, proliferation, apoptosis and cisplatin sensitivity through nuclear translocation of p-ATM and can be regulated by miR-145.

Article Snippet: Vector-sh-EXO1#2 (sc-44880-SH) was purchased from Santa Cruz Biotechnology.

Techniques: Functional Assay, Translocation Assay

Journal: Cell Cycle

Article Title: EXO1 overexpression is associated with poor prognosis of hepatocellular carcinoma patients

doi: 10.1080/15384101.2018.1534511

Figure Lengend Snippet: Analyses of the EXO1 gene in 143 HCC tissues.

Article Snippet: Exo1 shRNA (sc-44,880-SH) and its negative control carrying an irrelevant nucleotide (sc-108,060) were purchased from Santa Cruz Biotechnology (California, USA).

Techniques:

Journal: Cell Cycle

Article Title: EXO1 overexpression is associated with poor prognosis of hepatocellular carcinoma patients

doi: 10.1080/15384101.2018.1534511

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses of EXO1 mRNA expression and clinical variables for overall survival in HCC patients.

Article Snippet: Exo1 shRNA (sc-44,880-SH) and its negative control carrying an irrelevant nucleotide (sc-108,060) were purchased from Santa Cruz Biotechnology (California, USA).

Techniques: Expressing

Journal: Cell Cycle

Article Title: EXO1 overexpression is associated with poor prognosis of hepatocellular carcinoma patients

doi: 10.1080/15384101.2018.1534511

Figure Lengend Snippet: Analyses of the EXO1 gene in 143 HCC tissues.

Article Snippet: ShRNA, cell transfection and selection Exo1 shRNA (sc-44,880-SH) and its negative control carrying an irrelevant nucleotide (sc-108,060) were purchased from Santa Cruz Biotechnology (California, USA).

Techniques:

Journal: Cell Cycle

Article Title: EXO1 overexpression is associated with poor prognosis of hepatocellular carcinoma patients

doi: 10.1080/15384101.2018.1534511

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses of EXO1 mRNA expression and clinical variables for overall survival in HCC patients.

Article Snippet: ShRNA, cell transfection and selection Exo1 shRNA (sc-44,880-SH) and its negative control carrying an irrelevant nucleotide (sc-108,060) were purchased from Santa Cruz Biotechnology (California, USA).

Techniques: Expressing